Steam (moist heat) sterilization is commonly used for high heat compatible devices both for terminal sterilization and sterilization of reusable devices during reprocessing. LSO has the distinction of being the only Contract Steam Sterilization provider on the West Coast and maintains 4 validated autoclave chambers of various sizes and capabilities. (See autoclave chamber matrix ). Unique among these chambers is our 74 cf GmP autoclove with air-over-pressure capabilities for sterilizing flexible liquid containers such as blood and solution bags, syringes and vials.
Steam sterilization validations can be performed to various standards, including ISO 17665, EN285, AAMI ST79, etc. Both gravity and pre-vacuum cycles are performed at temperatures up to 135 C. LSO also performs biological indicator sterility testing in-house and offers the shortest lead times in the industry.
As part of any sterilization validation or production sterilization project, LSO offers:

Aesepticlave 1204V-AO
Chamber Vol: | ~74ft³ 2 M³ |
Chamber Size: Cart Size: Tray Size: |
36″ W x 84″ L x 42″ H 30″ W x 78″ L x 34″ H 15″ W x 39″ L x 1.75″ H |
Standards Performed: | EN285 ISO 17665 |
Cycle Types: | Gravity, Air-Over Gravity Pre-vac Leak Test Bowie Dick Drying |
Cycle Parameter Ranges |
|
Temperture (°C): | 121 to 135 |
Exposure Time: | 3 min to 4 hours |
Dry Time: | up to 4 hours |
Applications: | Liquid filled containers Large quantity device runs Large devices |

Getinge GE 6612 AR2
Chamber Vol: | ~19ft³ .5 M³ |
Chamber Size: Cart Size: Basket Size: |
26″ W x 49″ L x 26″ H 23″ W x 45″ L x 21.5″ H 19″ W x 22″ L x 8.5″ H |
Standards Performed: | ISO 17665 |
Cycle Types: | Gravity Pre-vac Leak Test Bowie Dick Drying |
Cycle Parameter Ranges |
|
Temperture (°C): | 121 to 128 |
Exposure Time: | 15 min to 2 hours |
Dry Time: | up to 2 hours |
Applications: | Small quantity device runs Medium-sized devices Kitted Devices R & D runs |

Market Forge STM-E
Chamber Vol: | ~5ft³ .1 M³ |
Chamber Size: Shelf Size: |
16″ dia x 26″ L 12″ W x 20″ L x 4″H |
Standards Performed: | AAMI ST79 |
Cycle Types: | Gravity Variable Exhaust Drying |
Cycle Parameter Ranges |
|
Temperture (°C): | 121 |
Exposure Time: | 15 min to 2 hours |
Dry Time: | up to 2 hours |
Applications: | Surgical Kits Reuseable devices Lab supplies Wrapped loads |

AMSCO EAGLE 3013
Chamber Vol: | ~3.9ft³ < .1 M³ |
Chamber Size: | 16″ W x 26″ L x 16″ H |
Standards Performed: | ISO 17664 AAMI ST79 |
Cycle Types: | Pre-Vac Gravity Drying |
Cycle Parameter Ranges |
|
Temperture (°C): | 121 to 135 |
Exposure Time: | 2 to 60 min |
Dry Time: | up to 1 hour |
Applications: | Surgical Kit Reusable Devices Lab Supplies Wrapped Loads R&D Runs |